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Materials and methods Experimental design Two experiments were conducted independently; one within Pfizer and the other within Vium animal facilities. Vium digital vivarium technology Vium Digital Smart Cages were outfitted with sensors that stream animal data and environmental conditions 24 hours a day, 7 days a week to a secure cloud-based data infrastructure [ 44 ].
Histological evaluation of DRG and peripheral nerve. Results and discussion General toxicity of paclitaxel Paclitaxel-dosed JAX mice showed significant weight loss starting from day 7 Download: PPT. Fig 1.
Body weight and food consumption changes following paclitaxel administration in JAX mice. Cold allodynia None of the vehicle-treated JAX mice responded to the cold stimuli within the 15 sec cut-off time when tested predose day-1 and on days 5, 12, and Table 1. Group incidences with severities of microscopic findings in JAX mice.
Table 2. Group incidences with severities of microscopic findings in CRL mice. Conclusions CiPN is a serious and common adverse effect in patients treated with chemotherapeutic agents for which there is a need for more consistent, automated, and clinically relevant methods to routinely assess toxicity.
Supporting information. S1 File. PCR data for Fig 4. S2 File. PCR data for Fig 5. S3 File. Body weight comparison of CRL mice. S4 File. Body weight data of CRL mice. Body weight raw data of CRL mice administered paclitaxel or vehicle. S5 File. Body weight data of JAX mice.
Cellular microRNA detection with miRacles
Body weight raw data and graph of JAX mice administered paclitaxel or vehicle. Acknowledgments We gratefully acknowledge Hayley Pryski and Timothy Coskran for their excellent technical assistance in tissue collection and processing and Kristin Edwards for editorial assistance. References 1. Chemotherapy-evoked painful peripheral neuropathy. Pain medicine. Chemotherapy-induced peripheral neuropathy. Oncology nursing forum. Diagnosis, management, and evaluation of chemotherapy-induced peripheral neuropathy. Seminars in oncology.
Wilkes G. Peripheral neuropathy related to chemotherapy. Seminars in oncology nursing. A review on oxaliplatin-induced peripheral nerve damage. Cancer treatment reviews. Healthcare costs and workloss burden of patients with chemotherapy-associated peripheral neuropathy in breast, ovarian, head and neck, and nonsmall cell lung cancer. Chemotherapy research and practice. Neurotoxic effects of antineoplastic drugs: the lesson of pre-clinical studies. Frontiers in bioscience: a journal and virtual library. Cavaletti G, Marmiroli P. Chemotherapy-induced peripheral neurotoxicity. Expert opinion on drug safety.
Bhagra A, Rao RD. Chemotherapy-induced neuropathy. Current oncology reports. Windebank AJ, Grisold W. Journal of the peripheral nervous system: JPNS. Starobova H, Vetter I. Pathophysiology of Chemotherapy-Induced Peripheral Neuropathy. Front Mol Neurosci. Therapy for chemotherapy-induced peripheral neuropathy. Peripheral neuropathies from chemotherapeutics and targeted agents: diagnosis, treatment, and prevention. Peripheral neuropathy with microtubule inhibitor containing antibody drug conjugates: Challenges and perspectives in translatability from nonclinical toxicology studies to the clinic.
Regulatory toxicology and pharmacology: RTP. Electrophysiologic assessment of sciatic nerve regeneration in the rat: surrounding limb muscles feature strongly in recordings from the gastrocnemius muscle. Journal of neuroscience methods. Dyer RS. The use of sensory evoked potentials in toxicology. Fundamental and applied toxicology: official journal of the Society of Toxicology.
Tactile allodynia in the absence of C-fiber activation: altered firing properties of DRG neurons following spinal nerve injury. Axotomy-induced miR promotes axon growth in adult dorsal root ganglion neurons. PloS one. Profile of microRNAs following rat sciatic nerve injury by deep sequencing: implication for mechanisms of nerve regeneration.
MicroRNAs , , and are down-regulated in primary neurons responding to sciatic nerve transection. Brain research.
Data Normalization Strategies for MicroRNA Quantification
Early changes of microRNAs expression in the dorsal root ganglia following rat sciatic nerve transection. Neuroscience letters. Dynamic changes in the microRNA expression profile reveal multiple regulatory mechanisms in the spinal nerve ligation model of neuropathic pain. Sources of individual variability: miRNAs that predispose to neuropathic pain identified using genome-wide sequencing.
Mol Pain. MicroRNA machinery responds to peripheral nerve lesion in an injury-regulated pattern. Front Genet. FEBS J. Soreq H, Wolf Y.
2. Optimized RT-PCR Reagents for miRNA Detection (TaqMan® MicroRNA Reverse Transcription Kit)
NeurimmiRs: microRNAs in the neuroimmune interface. Trends Mol Med.
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